Endocrine Abstracts

The chemically induced disturbance during the neurodevelopment stage could cause a serious disease. In vivo study is useful to discriminate against neurotoxic substances but is time-consuming and ethically problematic. So, we have previously established, developmental neurotoxicity test (DNT) in vitro method using Sox1-GFP. In this study we aimed to increase the predictability of discriminant function. The equation was statistically improved using thirty addi...

Hypoxia causes oxidative stress and is known to affect cardiovascular dysfunction and the programming of cardiovascular disease. Melatonin is the hormone released primarily from the pineal gland and has been proven to be an antioxidant. Melatonin promotes the expression of antioxidant enzymes such as superoxide dismutase and catalase. To confirm the effect of hypoxia on the differentiation of mouse embryonic stem cells (mESCs) into cardiomyocytes, hypoxia condition induced dur...

Developmental toxicity tests have been made by embryonic stem cell tests at the European Centre for the Validation of Alternative Methods or by embryonic body test in our laboratory. However, no neuronal-specific developmental toxicity test has been made yet. Therefore, this study was carried out using a 46C cell line, mouse embryonic stem cells with an endogenous Sox1-GFP reporter, to exploit the developmental neurotoxicity test. The expression of Sox1, a marker for neural pr...

Ki-67 can be solely detected within the cell nucleus, whereas in mitosis, most of the Ki-67 proteins are located on the chromosome surface. Ki-67 is present during all phases of the cell cycle (G1, S, G2, and M), but BrdU is only present in the S phase. This study examined whether it is possible to establish a developmental neurotoxicity test in human neural progenitor cells using Ki-67 instead of BrdU (5-bromo-2’-deoxyuridine). In the present study, Ki-67-expressed ReNce...

Developmental toxicity tests have been made by embryonic stem cell tests at the European Centre for the Validation of Alternative Methods or by embryonic body test in our laboratory. However, no neuronal-specific developmental toxicity test has been made yet. Therefore, this study was carried out using a 46C cell line, mouse embryonic stem cells with an endogenous Sox1-GFP reporter, to exploit the developmental neurotoxicity test. The expression of Sox1, a marker for neural pr...

Mitochondria, which are essential organelles for endocrine health, plays an important role in various physiological functions including hormonal biosynthesis, cell metabolism, proliferation and differentiation. Thus, mitochondrial toxicity can affect a variety of organs, such as liver, heart, muscle, kidney, and central nervous system. Mitochondrial toxicity is recognized as a contributor to drug-induced toxicity of various drugs such as hydroxytamoxifen, valproic acid, acetam...

Mitochondria play a key role in hormone biosynthesis and involve in renal cell proliferation by producing about 90% of cellular energy and controlling cell apoptosis. Doxorubicin is commonly used for many tumor treatments. However, its clinical utility is limited by the adverse reactions, which are known to be nephrotoxic. The mechanism by which doxorubicin induced kidney damage is still not completely understood, however nephrotoxicity by doxorubicin might be related to mitoc...

There have been raising concerns in the effects of endocrine disrupting chemicals like triclosan (TCS) on the embryo development. Triclosan (TCS) is commonly present in household and personal wash products We hypothesize that exposure to TCS during early stage of development could alter brain development affecting behavior. To test this hypothesis, primary cortical neurons were exposure to TCS with/without estrogen antiestrogen ICI 182780 from day in vitro 1 to 4. We also addr...